Journal: Frontiers in Immunology

Article Title: Unveiling B7/CD28 family proteins in hepatocellular carcinoma: insights into communication and prognostic significance

doi: 10.3389/fimmu.2025.1583597

Figure Lengend Snippet: The expression pattern of B7H3 in tumor tissues. (A) The representative IHC images of Isotype control antibody and B7H3 antibody; (B) The representative IHC images of diffuse HCC and well-circumscribed HCC; (C) Representative multi-immunofluorescence images of AFP (green) and B7H3 (red). The nucleus is labeled with DAPI (blue). (D) Proportions of B7H3 on tumor cells (TCs), in stroma and in both. (E) The representative IHC images of B7H3 from serial sections. (F) OS and PFS for HCC patients based on the expression of B7H3 on tumor tissue. (G) OS and PFS for HCC patients based on the expression of B7H3 in the stroma and on tumor cells.

Article Snippet: In the end, the specimens were prepared by applying ProLongTM Diamond Antifade mountant with DAPI (G1236, Servicebio, Wuhan, China).

Techniques: Expressing, Control, Immunofluorescence, Labeling

Journal: bioRxiv

Article Title: CSRefiner: A lightweight framework for fine-tuning cell segmentation models with small datasets

doi: 10.1101/2025.09.14.675731

Figure Lengend Snippet: (A) Representative segmentation results from four pre-trained models (Cellpose-cyto, Cellpose-cpsam, StarDist-2D_versatile_fluo, and CellBin) for FFPE mouse brain tissue with DAPI-stained. Four regions are shown, including hippocampal and non-hippocampal areas. Red contours indicate ground-truth manual annotations, and yellow contours indicate model-predicted segmentation boundaries, the corresponding F1 scores are labeled for quantification. (B) Schematic overview of the CSRefiner workflow, including training set preparation, model selection and fine-tuning, performance evaluation, and downstream generation of CellBin GEF files. (C) Segmentation results after fine-tuning with CSRefiner, corresponding to the regions shown in panel A.

Article Snippet: FFPE DAPI-stained mouse brain section: Fresh mouse brains were dissected and fixed in 4% paraformaldehyde (Servicebio, G1121) at room temperature for 24-48 hours, followed by dehydration, paraffin embedding, and sectioning into 5 μ m-thick slices using standard histopathological protocols.

Techniques: Staining, Labeling, Selection